Into chromosome cuatro, most of the three QTLs was basically in the 66
Container plots of land showing the results of your own marker genotypic groups (AAAA, AAAB, AABB, ABBB, BBBB) of cuatro applicant SNPs to your tuber glucose quantity (GLU); bud-stop fry color (FBE); stem-tuber fry color (FSE); ID, Idaho
The brand new co-localization from numerous QTLs at readiness locus part within study is consistent with the noticed designs from phenotypic correlations. 01) with several traits overlapping an equivalent genetic standing (Fig. 1). For example, EBID11 and VWID11 scores each other demonstrated a robust bad correlation with MAT95ID and you may MAT120ID. That it development get give an explanation for major impact QTL with after readiness closely with the postponed start of VW and EB, or alternatively, early readiness leading to early start of VW and you can EB. A comparatively high correlation was also seen between vine readiness and you may increases habit, which have very early readiness associated with the even more distribute development behavior. In contrast, a minimal correlation (roentgen ? 0.2) ranging from vine readiness and you will each other tuber sugar concentration and you may bud-end fry colour is consistent with different types with the QTL effects.
Tuber glucose, bud-prevent and you will stem-avoid fry colour
As well as chromosome 5, high QTLs to possess sugar, bud-stop and you may base-end fry colour was in fact seen within the overlapping aspects of chromosomes 4, 6, ten, and you may 11. 0 cM to your LOD results anywhere between cuatro.five to six.0 (Table step three, Fig. 4). These QTLs said ranging from seven.dos and eleven.3% of your own characteristic difference. Data of various easy activities conveyed a single greatest-fitting model for everybody three attributes, that have a double-simplex allele (AAAB ? AAAB) into the homologous chromosomes H2 and you will H5 regarding RGR and Advertising, respectively (Desk step 3, More file 8: Contour S4). Getting sugar, the newest B allele are in the high sugar quantity, if you find yourself to possess bud-avoid and you will base-avoid fry color, the clear presence of the brand new B allele conveyed all the way down reflectance opinions (Fig. 3b).
LOD rating shipment of QTLs for a few traits along the twelve potato chromosomes (Chr). Abbreviations is such as Fig. step 1
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
eight Mb) with https://datingranking.net/escort-directory/davenport/ numerous genes on the starch/sugar inter-sales in addition to hexokinase (HXK-6, PGSC0003DMG400016521), fructokinase (FRK-6/step 1, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and you may invertase (INV-na-6/step one, PGSC0003DMG400026107, INV-6/2, PGSC0003DMG400033142) family genes.